Rat Neutrophils Express a 4 and b 1 Integrins and Bind to Vascular Cell Adhesion Molecule
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چکیده
The a4 integrins, which are constitutively expressed on all human leukocyte subtypes except neutrophils, interact with vascular cell adhesion molecule-1 (VCAM-1) and mucosal addressin cell adhesion molecule (MAdCAM-1) on endothelium to mediate selective recruitment of leukocyte subpopulations, other than neutrophils, to sites of inflammation. However, here we report that a different paradigm of leukocyte recruitment may exist in the rat. Flow cytometric analysis of rat neutrophils using a panel of monoclonal antibodies which recognize rat a4 and b1 integrins showed consistent, low levels of expression. Although a4 was expressed at lower levels on neutrophils than all other rat leukocytes, this level of expression was sufficient to mediate significant levels of a4and b1-dependent neutrophil adhesion to rat and human VCAM-1, and a4-dependent, but b1-independent, adhesion to human MAdCAM-1. These data suggest that rat neutrophils, unlike other species, may use a4 integrins to traffic to sites of inflammation in vivo. r 1998 by The American Society of Hematology.
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تاریخ انتشار 1998